Publicaciones por año
Publicaciones del Departamento de Histología y Embriología
Cell Proliferation, Migration, and Neurogenesis in the Adult Brain of the Pulse Type Weakly Electric Fish, Gymnotus omarorum
Front Neurosci 2017 11:437
Valentina Olivera-Pasilio 1 2 3 , Moira Lasserre 1 , María E Castelló 1 3
1 Desarrollo y Evolución Neural, Departamento de Neurociencias Integrativas y Computacionales, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y CulturaMontevideo, Uruguay. 2 Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la RepúblicaMontevideo, Uruguay. 3 IIBE "Histología de Sistemas Sensoriales", Unidad Asociada F. de MedicinaMontevideo, Uruguay.
DOI: 10.3389/fnins.2017.00437
PMID: 28860962
Pubmed: https://pubmed.ncbi.nlm.nih.gov/28860962
Texto completo: https://doi.org/10.3389/fnins.2017.00437
Abstract:
Adult neurogenesis, an essential mechanism of brain plasticity, enables brain development along postnatal life, constant addition of new neurons, neuronal turnover, and/or regeneration. It is amply distributed but negatively modulated during development and along evolution. Widespread cell proliferation, high neurogenic, and regenerative capacities are considered characteristics of teleost brains during adulthood. These anamniotes are promising models to depict factors that modulate cell proliferation, migration, and neurogenesis, and might be intervened to promote brain plasticity in mammals. Nevertheless, the migration path of derived cells to their final destination was not studied in various teleosts, including most weakly electric fish. In this group adult brain morphology is attributed to sensory specialization, involving the concerted evolution of peripheral electroreceptors and electric organs, encompassed by the evolution of neural networks involved in electrosensory information processing. In wave type gymnotids adult brain morphology is proposed to result from lifelong region specific cell proliferation and neurogenesis. Consistently, pulse type weakly electric gymnotids and mormyrids show widespread distribution of proliferation zones that persists in adulthood, but their neurogenic potential is still unknown. Here we studied the migration process and differentiation of newborn cells into the neuronal phenotype in the pulse type gymnotid Gymnotus omarorum. Pulse labeling of S-phase cells with 5-Chloro-2'-deoxyuridine thymidine followed by 1 to 180 day survivals evidenced long distance migration of newborn cells from the rostralmost telencephalic ventricle to the olfactory bulb, and between layers of all cerebellar divisions. Shorter migration appeared in the tectum opticum and torus semicircularis. In many brain regions, derived cells expressed early neuronal markers doublecortin (chase: 1-30 days) and HuC/HuD (chase: 7-180 days). Some newborn cells expressed the mature neuronal marker tyrosine hydroxylase in the subpallium (chase: 90 days) and olfactory bulb (chase: 180 days), indicating the acquisition of a mature neuronal phenotype. Long term CldU labeled newborn cells of the granular layer of the corpus cerebelli were also retrogradely labeled "in vivo," suggesting their insertion into the neural networks. These findings evidence the neurogenic capacity of telencephalic, mesencephalic, and rhombencephalic brain proliferation zones in G. omarorum, supporting the phylogenetic conserved feature of adult neurogenesis and its functional significance.
NLRP3 inflammasome-driven pathways in depression: Clinical and preclinical findings
Brain Behav Immun 2017 64:367-383
Fernanda N Kaufmann 1 , Ana Paula Costa 1 , Gabriele Ghisleni 2 , Alexandre P Diaz 3 , Ana Lúcia S Rodrigues 1 , Hugo Peluffo 4 , Manuella P Kaster 5
1 Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil. 2 Department of Life and Health Sciences, Catholic University of Pelotas, Rio Grande do Sul, Brazil. 3 Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Santa Catarina, Brazil. 4 Neuroinflammation and Gene Therapy Lab., Institut Pasteur de Montevideo, Uruguay; Dept. Histology and Embryology, Faculty of Medicine, UDELAR, Uruguay. 5 Department of Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil. Electronic address: manuella.kaster@ufsc.br.
DOI: 10.1016/j.bbi.2017.03.002
PMID: 28263786
Pubmed: https://pubmed.ncbi.nlm.nih.gov/28263786
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/S0889-1591(17)30064-8
Abstract:
Over the past three decades, an intricate interaction between immune activation, release of pro-inflammatory cytokines and changes in brain circuits related to mood and behavior has been described. Despite extensive efforts, questions regarding when inflammation becomes detrimental or how we can target the immune system to develop new therapeutic strategies for the treatment of psychiatric disorders remain unresolved. In this context, novel aspects of the neuroinflammatory process activated in response to stressful challenges have recently been documented in major depressive disorder (MDD). The Nod-like receptor pyrin containing 3 inflammasome (NLRP3) is an intracellular multiprotein complex responsible for a number of innate immune processes associated with infection, inflammation and autoimmunity. Recent data have demonstrated that NLRP3 activation appears to bridge the gap between immune activation and metabolic danger signals or stress exposure, which are key factors in the pathogenesis of psychiatric disorders. In this review, we discuss both preclinical and clinical evidence that links the assembly of the NLRP3 complex and the subsequent proteolysis and release of the pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) in chronic stress models and patients with MDD. Importantly, we also focus on the therapeutic potential of targeting the NLRP3 inflammasome complex to improve stress resilience and depressive symptoms.
Associations between male infertility and ancestry in South Americans: a case control study
BMC Med Genet 2017 18(1):78
Maria Fernanda Skowronek 1 , Tatiana Velazquez 2 , Patricia Mut 2 , Gonzalo Figueiro 3 , Monica Sans 3 , Bernardo Bertoni 2 , Rossana Sapiro 4
1 Departamento de Histología y Embriología, Facultad de Medicina UDELAR, Montevideo, Uruguay. 2 Departamento de Genética, Facultad de Medicina UDELAR, Montevideo, Uruguay. 3 Departamento de Antropología, Facultad de Humanidades y Ciencias de la Educación, UDELAR, Montevideo, Uruguay. 4 Departamento de Histología y Embriología, Facultad de Medicina UDELAR, Montevideo, Uruguay. rsapiro@fmed.edu.uy.
DOI: 10.1186/s12881-017-0438-z
PMID: 28747152
Pubmed: https://pubmed.ncbi.nlm.nih.gov/28747152
Texto completo: https://bmcmedgenet.biomedcentral.com/articles/10.1186/s12881-017-0438-z
Abstract:
Background: Infertility affects 15% of human couples, with men being responsible in approximately 50% of cases. Moreover, the aetiology of male factor infertility is poorly understood. The majority of male factor infertility remains idiopathic and potentially genetic in origin. The association of the Y chromosome and mitochondrial haplogroups with male infertility has been previously reported. This association differs between studied populations and their geographical distributions. These effects have been only rarely analysed in mixed populations, such as South Americans.
Methods: In this study, we analysed the contributions of the Y chromosome and mitochondrial haplogroups to male infertility in a mixed population. A case control study was conducted. Regular PCR and high-resolutionmelting- real-time PCR were performed to type haplogroups from fertile and infertile men. The sperm parameters from infertile men were compared in each haplogroup by logistic regression analysis and ANOVA.
Results: The genotyping confirmed the known admixture characteristic of the Uruguayan population. The European paternal contribution was higher than the maternal contribution in both fertile and infertile men. Neither maternal nor paternal ancestry presented differences between the cases and controls. Men belonging to the Y chromosome haplogroup F(xK) more frequently presented with an abnormal sperm morphology than men from other haplogroups. The sperm parameters were not associated with the mitochondrial haplogroups.
Conclusions: The data presented in this study showed an association between male infertility and ancestry in the Uruguayan population. Specifically, abnormal sperm morphology was associated with the Y chromosome haplogroup F(xK). Since the Y chromosome lacks recombination, these data suggest that some genes that determine sperm morphology might be inherited in blocks with the region that determines specific haplogroups. However, the possible association between the Y chromosome haplogroup F(xK) and sperm morphology requires further confirmatory testing. Data linking infertility with ancestry are needed to establish the possible causes of infertility and define male populations susceptible to infertility. Whether the admixed characteristics of the Uruguayan population exert any pressure on male fertility potential must be further investigated.
An immunohistochemical study on the distribution of vasotocin neurons in the brain of two weakly electric fish, Gymnotus omarorum and Brachyhypopomus gauderio
Tissue Cell 2017 49(2 Pt B):257-269
Paula Pouso 1 , Milka Radmilovich 2 , Ana Silva 3
1 Depto Histología y Embriología, Facultad de Medicina, Universidad de la República, Montevideo 11800, Uruguay; Unidad Bases Neurales de la Conducta, Departamento de Neurofisiología Celular y Molecular, IIBCE, Montevideo 11600, Uruguay. 2 Depto Histología y Embriología, Facultad de Medicina, Universidad de la República, Montevideo 11800, Uruguay. 3 Unidad Bases Neurales de la Conducta, Departamento de Neurofisiología Celular y Molecular, IIBCE, Montevideo 11600, Uruguay; Laboratorio de Neurociencias, Facultad de Ciencias, Universidad de la República, Montevideo 11400, Uruguay. Electronic address: asilva@fcien.edu.uy.
DOI: 10.1016/j.tice.2017.02.003
PMID: 28242105
Pubmed: https://pubmed.ncbi.nlm.nih.gov/28242105
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/S0040-8166(16)30317-2
Abstract:
Hypothalamic nonapeptides (arginin vasotocin-vasopressin, oxytocin-isotocin) are known to modulate social behaviors across vertebrates. The neuroanatomical conservation of nonapeptide systems enables the use of novel vertebrate model species to identify general strategies of their functional mechanisms. We present a detailed immunohistochemical description of vasotocin (AVT) cell populations and their projections in two species of weakly electric fish with different social structure, Gymnotus omarorum and Brachyhypopomus gauderio. Strong behavioral, pharmacological, and electrophysiological evidence support that AVT modulation of electric behavior differs between the gregarious B. gauderio and the solitary G. omarorum. This functional diversity does not necessarily depend on anatomical differences of AVT neurons. To test this, we focus on interspecific comparisons of the AVT system in basal non-breeding males along the brain. G. omarorum and B. gauderio showed similar AVT somata sizes and comparable distributions of AVT somata and fibers. Interestingly, AVT fibers project to areas related to the control of social behavior and electromotor displays in both species. We found that no gross anatomical differences in the organization of the AVT system account for functional differences between species, which rather shall depend on the pattern of activation of neurons embedded in the same basic anatomical organization of the AVT system.
Focal Transplantation of Aberrant Glial Cells Carrying the SOD1G93A Mutation into Rat Spinal Cord Induces Extensive Gliosis
Neuroimmunomodulation 2017 24(3):143-153
Sofía Ibarburu 1 , Emiliano Trias 1 , Natalia Lago 2 , Hugo Peluffo 2 3 , Romina Barreto-Núñez 1 , Valentina Varela 1 , Joseph S Beckman 4 , Luis Barbeito 1
1 Laboratorio de Neurodegeneración, Institut Pasteur de Montevideo, 2 Laboratorio de Neuroinflamación y Terapia Génica, Institut Pasteur de Montevideo, and 3 Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; 4 Linus Pauling Institute, Oregon State University, Corvallis, OR, USA
DOI: 10.1159/000480639
PMID: 29131016
Pubmed: https://pubmed.ncbi.nlm.nih.gov/29131016
Texto completo: https://doi.org/10.1159/000480639
Abstract:
Objective: We aimed to determine the potential of aberrant glial cells (AbAs) isolated from the spinal cord of adult SOD1G93A symptomatic rats to induce gliosis and neuronal damage following focal transplantation into the lumbar spinal cord of wild-type rats.
Methods: AbAs were obtained from the spinal cords of SOD1G93A symptomatic rats. One hundred thousand cells were injected using a glass micropipette into the lumbar spinal cords (L3-L5) of syngeneic wild-type adult rats. Equal volumes of culture medium or wild-type neonatal microglia were used as controls. Seven days after transplantation, immunohistochemistry analysis was carried out using astrocytic and microglia cell markers. Transplanted SOD1G93A AbAs were recognized by specific antibodies to human SOD1 (hSOD1) or misfolded human SOD1.
Results: Seven days after transplantation, AbAs were mainly detected in the medial region of the lumbar ventral horn as a well-limited cell cluster formed at the site of injection by their immunoreactivity to either misfolded SOD1 or normally folded hSOD1. Compared with controls, transplanted AbAs were surrounded by marked microgliosis and reactive astrocytes. Marked microgliosis was observed to extend bilaterally up to the cervical cord. Motor neurons close to AbA transplants were surrounded by activated glial cells and displayed ubiquitin aggregation.
Conclusions: AbAs bearing mutant SOD1G93A have the potential to induce neuroinflammation along the spinal cord and incipient damage to the motor neurons. The emergence of AbAs during amyotrophic lateral sclerosis pathogenesis may therefore be a mechanism to boost neuroinflammation and spread motor neuron damage along the neuroaxis.
Astrocytic Expression of the Immunoreceptor CD300f Protects Hippocampal Neurons from Amyloid-β Oligomer Toxicity In Vitro
Curr Alzheimer Res 2017 14(7):778-783
Thiago Zaqueu Lima 1 , Luis Roberto Sardinha 2 , Joan Sayos 3 , Luiz Eugenio Mello 1 , Hugo Peluffo 4
1 Department of Physiology, Universidade Federal de Sao Paulo, Sao Paulo. Brazil. 2 Hospital Israelita Albert Einstein, Sao Paulo. Brazil. 3 Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBBER-BBN), Instituto de Salud Carlos III, Barcelona. Spain. 4 Departamento de Histología y Embriología, Facultad de Medicina, UDELAR, Montevideo, Uruguay; Neuroinflammation and Gene Therapy Laboratory, Institut Pasteur de Montevideo. Uruguay.
DOI: 10.2174/1567205014666170202121709
PMID: 28155597
Pubmed: https://pubmed.ncbi.nlm.nih.gov/28155597
Texto completo: https://www.eurekaselect.com/article/81448
Abstract:
Background: Astrocytes contribute to neuroinflammation that accompanies neurodegenerative disorders such as Alzheimer's disease (AD). In this sense, the toxicity of these diseases might be attenuated through the modulation of astrocytic inflammatory responses. Recently, the CD300f immunoreceptor was described as a new member of the CD300 immunoreceptor family, showing promising modulatory properties.
Objective: Here, we investigated whether overexpression of hCD300f (the human isoform of CD300f) in astrocytes protects hippocampal neurons against the degeneration induced by amyloid-beta (Aβ) oligomer.
Method: Astrocyte monolayers were transfected with hCD300f before seeding the hippocampal neurons, and then the co-culture was exposed to Aβ1-42 oligomers (5 μM, 48h).
Results: hCD300f expression significantly abrogated the neuronal loss elicited by Aβ. This effect was dependent on neuron-astrocyte cell-cell interactions since no protection was observed using conditioned media from transfected astrocytes. Astrocyte modulation was dependent on the cytoplasmic signaling tail of hCD300f. Furthermore hCD300f expression did not affect the ability of astrocytes to uptake Aβ1- 42 oligomers by endocytosis, which discards the possibility that increased Aβ1-42 clearance could mediate neuroprotection by hCD300f.
Conclusion: These results suggest that the astrocyte-directed expression of the hCD300f immune receptor can be a neuroprotective strategy in AD disease.
Post-hatching brain morphogenesis and cell proliferation in the pulse-type mormyrid Mormyrus rume proboscirostris
J Physiol Paris 2016 110(3 Pt B):245-258
Milka Radmilovich 1 , Isabel Barreiro 2 , Leticia Iribarne 3 , Kirsty Grant 4 , Frank Kirschbaum 5 , María E Castelló 6
1 Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay; Unidad Asociada "Histología de Sistemas Sensoriales", Facultad de Medicina-Instituto de Investigaciones Biológicas Clemente Estable, Uruguay. Electronic address: milka@fmed.edu.uy. 2 Unidad Asociada "Histología de Sistemas Sensoriales", Facultad de Medicina-Instituto de Investigaciones Biológicas Clemente Estable, Uruguay; Desarrollo y Evolución Neural, Departamento de Neurociencias Integrativas y Computacionales, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay. Electronic address: isabelpepis@gmail.com. 3 Desarrollo y Evolución Neural, Departamento de Neurociencias Integrativas y Computacionales, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay. Electronic address: irilet@gmail.com. 4 Unit of Neuroscience Information and Complexity, CNRS FRE, 3693 Gif-sur-Yvette, France. Electronic address: kirsty.grant.unic@gmail.com. 5 Unit Biology and Ecology of Fishes, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, Germany. Electronic address: frank.kirschbaum@staff.hu-berlin.de. 6 Unidad Asociada "Histología de Sistemas Sensoriales", Facultad de Medicina-Instituto de Investigaciones Biológicas Clemente Estable, Uruguay; Desarrollo y Evolución Neural, Departamento de Neurociencias Integrativas y Computacionales, Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura, Montevideo, Uruguay. Electronic address: mcastello@iibce.edu.uy.
DOI: 10.1016/j.jphysparis.2016.11.007
PMID: 27888101
Pubmed: https://pubmed.ncbi.nlm.nih.gov/27888101
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/S0928-4257(16)30029-8
Abstract:
The anatomical organization of African Mormyrids' brain is a clear example of departure from the average brain morphotype in teleosts, probably related to functional specialization associated to electrosensory processing and sensory-motor coordination. The brain of Mormyrids is characterized by a well-developed rhombencephalic electrosensory lobe interconnected with relatively large mesencephalic torus semicircularis and optic tectum, and a huge and complex cerebellum. This unique morphology might imply cell addition from extraventricular proliferation zones up to late developmental stages. Here we studied the ontogeny of these brain regions in Mormyrus rume proboscirostris from embryonic to adult stages by classical histological techniques and 3D reconstruction, and analyzed the spatial-temporal distribution of proliferating cells, using pulse type BrdU labeling. Brain morphogenesis and maturation progressed in rostral-caudal direction, from 4day old free embryos, through larvae, to juveniles whose brain almost attained adult morphological complexity. The change in the relative size of the telencephalon, and mesencephalic and rhombencephalic brain regions suggest a developmental shift in the relative importance of visual and electrosensory modalities. In free embryos, proliferating cells densely populated the lining of the ventricular system. During development, ventricular proliferating cells decreased in density and extension of distribution, constituting ventricular proliferation zones. The first recognizable one was found at the optic tectum of free embryos. Several extraventricular proliferation zones were found in the cerebellar divisions of larvae, persisting along life. Adult M. rume proboscirostris showed scarce ventricular but profuse cerebellar proliferation zones, particularly at the subpial layer of the valvula cerebelli, similar to lagomorphs. This might indicate that adult cerebellar proliferation is a conserved vertebrate feature.
Ovarian follicular dynamics after aromatizable or non aromatizable neonatal androgenization
J Mol Histol 2016 47(5):491-501
Gabriel Anesetti 1 , Rebeca Chávez-Genaro 2
1 Histology and Embryology Department, School of Medicine, General Flores 2125, CP 11800, Montevideo, Uruguay. ganesett@fmed.edu.uy. 2 Histology and Embryology Department, School of Medicine, General Flores 2125, CP 11800, Montevideo, Uruguay.
DOI: 10.1007/s10735-016-9692-1
PMID: 27541036
Pubmed: https://pubmed.ncbi.nlm.nih.gov/27541036
Texto completo: https://doi.org/10.1007/s10735-016-9692-1
Abstract:
The effects of neonatal testosterone or dihydrotestosterone exposure on ovarian follicular dynamics were analysed at prepubertal, pubertal or adult age in Wistar rats. Both androgens induced a transitory increase on follicular endowment that was partially corrected at puberty. At adult age testosterone prevented ovulation, without significant modifications on follicular dynamics. An increased number of cystic structures were observed from puberty to adult age. However, ovaries of rats treated with dihydrotestosterone showed follicles with evident morphological alterations in granulosa and thecal layers although several corpora lutea were observed. A significant increase in preantral follicles and few cystic structures were detected at advanced adulthood. The size of cyst increased with age. No immunohistochemical changes on growth factors or enzymes related to steroidogenesis in growing follicles were obvious in any group. In both androgenized groups, cysts shared immunohistochemical characteristics exhibited by preovulatory follicles but they were unable to ovulate spontaneously. Our results provide an insight into the role of different androgens in female reproductive system development, indicating a direct effect of dihydrotestosterone on ovarian tissues whereas a central effect would be the main feature of neonatal testosterone exposure. Heterogeneous clinical manifestations seen in pathologies such as polycystic ovary syndrome among women could be associated with subtle hormonal changes during follicular population development.
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-α/β1-Independent
PLoS One 2016 11(8):e0162033
Belén Torrado 1 , Martín Graña 2 , José L Badano 1 , Florencia Irigoín 1 3
1 Human Molecular Genetics Laboratory, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo CP11400, Uruguay. 2 Bioinformatics Unit, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo CP11400, Uruguay. 3 Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo CP11800, Uruguay.
DOI: 10.1371/journal.pone.0162033
PMID: 27579771
Pubmed: https://pubmed.ncbi.nlm.nih.gov/27579771
Texto completo: https://dx.plos.org/10.1371/journal.pone.0162033
Abstract:
Gli2 is the primary transcriptional activator of Hedgehog signalling in mammals. Upon stimulation of the pathway, Gli2 moves into the cilium before reaching the nucleus. However, the mechanisms underlying its entry into the cilium are not completely understood. Since several similarities have been reported between nuclear and ciliary import, we investigated if the nuclear import machinery participates in Gli2 ciliary entry. Here we show that while two conserved classical nuclear localization signals mediate Gli2 nuclear localization via importin (Imp)-α/β1, these sequences are not required for Gli2 ciliary import. However, blocking Imp-mediated transport through overexpression of GTP-locked Ran reduced the percentage of Gli2 positive cilia, an effect that was not explained by increased CRM1-dependent export of Gli2 from the cilium. We explored the participation of Imp-β2 in Gli2 ciliary traffic and observed that this transporter is involved in moving Gli2 into the cilium, as has been described for other ciliary proteins. In addition, our data indicate that Imp-β2 might also collaborate in Gli2 nuclear entry. How does Imp-β2 determine the final destination of a protein that can localize to two distinct subcellular compartments remains an open question. Therefore, our data shows that the nuclear-cytoplasmic shuttling machinery plays a critical role mediating the subcellular distribution of Gli2 and the activation of the pathway, but distinct importins likely play a differential role mediating its ciliary and nuclear translocation.
A Natural Cattle Immune Response Against Horn Fly (Diptera: Muscidae) Salivary Antigens May Regulate Parasite Blood Intake
J Econ Entomol 2016 109(4):1951-6
M Breijo 1 , L Pastro 2 , S Rocha 3 , X Ures 3 , P Alonzo 3 , M Santos 3 , C Bolatto 4 , C Fernández 5 , A Meikle 6
1 Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy) mbreijo@fmed.edu.uy. 2 Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay (lpastro@fcien.edu.uy). 3 Unidad de Reactivos y Biomodelos de Experimentación, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (mbreijo@fmed.edu.uy; gabrielrocha85@gmail.com; ximenaures@gmail.com; palonzo@higiene.edu.uy; msantos@fmed.edu.uy). 4 Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Gral. Flores 2125, Montevideo, Uruguay (cbolatto@fmed.edu.uy). 5 Cátedra de Inmunología, Facultad de Química, Instituto de Higiene, Universidad de la República, Av. Alfredo Navarro 3051, 11600 Montevideo, Uruguay (cfernan@fq.edu.uy). 6 Laboratorio de Técnicas Nucleares, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, Montevideo, Uruguay (meikleana@gmail.com).
DOI: 10.1093/jee/tow133
PMID: 27329632
Pubmed: https://pubmed.ncbi.nlm.nih.gov/27329632
Texto completo: https://academic.oup.com/jee/article-lookup/doi/10.1093/jee/tow133
Abstract:
The horn fly, Haematobia irritans (L.), is a blood-sucking ectoparasite that is responsible for sizeable economic losses in livestock. The salivary gland products facilitate blood intake. Taking advantage of the identification of novel H. irritans salivary antigens (Hematobin, HTB and Irritans 5, IT5), we investigated the parasite loads, H. irritans blood intake, and antibody response of naturally infected bovines during the fly season. Fly loads and fly hemoglobin content fluctuated during the trial. Each time horn fly loads exceeded 200 flies per cattle, a reduction in horn fly blood intake was observed three weeks later. All of the cattle elicited an antibody response against HTB and IT5 that declined once the fly season was over. Cattle anti-IT5 titers were positively correlated with parasite loads and negatively correlated with fly blood intake. These results suggest that the natural changes in the H. irritans blood intake observed in this study were associated with a natural host response against horn fly salivary antigens.