Steroid-producing cells in chick intersexual gonads

Gen Comp Endocrinol 1970 Feb;14(1):164-9

R Narbaitz, E M De Robertis Jr

a Cleft Palate Research Center, University of Pittsburgh USA b Departamento de Histología y Embriología, Facultad de Medicina, Montevideo, Uruguay

DOI: 10.1016/0016-6480(70)90020-1
PMID: 5461621
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5461621
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/0016-6480(70)90020-1

Abstract:
Eggs of a sex-linked cross were injected on the fourth day of incubation with 100 μg of estradiol benzoate. Both injected and control chicks were sacrificed at varying intervals after hatching and a histochemical study of their gonads was conducted. Medullary cells, rich in lipids and with a high 3β-hydroxysteroid activity, were present in newly hatched inverted embryos with a localization similar to that found in normal female gonads. These characterstics persisted in the inverted gonad throughout the whole period of sex reversion. As these cells are supposed to secrete estrogens, it is suggested that the regression of cortex in inverted gonads is not due to lack of estrogen stimulation. Rapid proliferation and differentiation of testicular tissue in the medullary region takes place after the cortex has started its involution and appears to be a consequence of this process, rather than a cause.

narbaitz1970.pdf

Effects of hemispaying on guinea pigs injected with testosterone on the first day of life

Acta Physiol Lat Am 1969 19(4):315-23

E Carlevaro, L Riboni, H D'Albora, R Domínguez, W Buño

DOI:
PMID: 5393647
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5393647
Texto completo:

Abstract:

Postnatal evolution of steroidogenic cells in the chick ovary

Histochemie 1968 15(3):187-93

R Narbaitz, E M De Robertis Jr

Departamento de Histologla y Embriologia, Facultad de Medicina, Montevideo, Uruguay

DOI: 10.1007/BF00305882
PMID: 4246253
Pubmed: https://pubmed.ncbi.nlm.nih.gov/4246253
Texto completo: https://link.springer.com/article/10.1007/BF00305882

Abstract:
Summary. From hatching to sexual maturity, the left ovary of the chick was studied with cytochemical techniques for the demonstration of steroid synthesis. At the time of hatching, there are in the ovarian medulla cell groups loaded with lipids and cholesterol which give a positive reaction for Ah-3fl-hydroxysteroid dehydrogenase (Ah-3fl-HSDH). Because of the predominant growth of the cortex in the course of the postnatal morphogenesis of the ovary, these cells are shifted to the theca interna of the follicles and the interfollicular spaces (interstitial cells). The cytochemical tests used demonstrate that, at all the stages studied, there is steroid synthesis in these cells. From the 20th day on, granulosa cells show an unspecific enzymatic reaction, which is positive in the control sections incubated without substrate. A strong As-3fl-HSDH activity is observed in the granulosa cells of postovulatory follicles. The significance of these findings in relation to the life history of steroid producing cells in the embryonic and postnatal chick ovary is discussed.

1968 de robertis.pdf

SITES OF ESTERASE ACTIVITY IN THE GUINEA PIG EMBRYO

Acta Anat 1965 60:285-97

Washington Buño

Department of Histology and Embryology, Facultad de Medicina. Montevideo, Uruguay

DOI: 10.1159/000142654
PMID: 14314655
Pubmed: https://pubmed.ncbi.nlm.nih.gov/14314655
Texto completo: https://karger.com/aan/article-abstract/60/2/285/5926/SITES-OF-ESTERASE-ACTIVITY-IN-THE-GUINEA-PIG?redirectedFrom=fulltext

Abstract:

Buño 1965.pdf