Localization of basic fibroblast growth factor binding sites in the chick embryonic neural retina

Differentiation 1990 Dec;45(3):161-7

A Cirillo 1 , C Arruti 1 2, Y Courtois 3, J C Jeanny 3

1 Laboratorio de Cultivo de Tejidos, Depto de Histología y Embriología, Facultad de Medicina, Avda General Flores 2125, 11800 Montevideo, Uruguay 2 Depto de Biologia Celular, Facultad de Humanidades y Ciencias, Tristin Narvaja 1464, 11200 Montevideo, Uruguay 3 Unité de Recherches Gérontologiques, INSERM U. 118, Unité Affilié CNRS, 29, rue Wilhem, F-75016 Paris, France

DOI: 10.1111/j.1432-0436.1990.tb00469.x
PMID:
Pubmed: https://pubmed.ncbi.nlm.nih.gov/
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/S0301-4681(11)60217-X

Abstract:
We have investigated the localization of basic fibroblast growth factor (bFGF) binding sites during the development of the neural retina in the chick embryo. The specificity of the affinity of bFGF for its receptors was assessed by competition experiments with unlabelled growth factor or with heparin, as well as by heparitinase treatment of the samples. Two different types of binding sites were observed in the neural retina by light-microscopic autoradiography. The first type, localized mainly to basement membranes, was highly sensitive to heparitinase digestion and to competition with heparin. It was not developmentally regulated. The second type of binding site, resistant to heparin competition, appeared to be associated with retinal cells from the earliest stages studied (3-day-old embryo, stages 21-22 of Hamburger and Hamilton). Its distribution was found to vary during embryonic development, paralleling layering of the neural retina. Binding of bFGF to the latter sites was observed throughout the retinal neuroepithelium at early stages but displayed a distinct pattern at the time when the inner and outer plexiform layers were formed. During the development of the inner plexiform layer, a banded pattern of bFGF binding was observed. These bands, lying parallel to the vitreal surface, seemed to codistribute with the synaptic bands existing in the inner plexiform layer. The presence of intra-retinal bFGF binding sites whose distribution varies with embryonic development suggests a regulatory mechanism involving differential actions of bFGF on neural retinal cells.

The effect of eye-derived-growth-factor (EDGFs) on methionine incorporation in the different cell populations of bovine adult lens in organ culture

Exp Eye Res 1989 48(2):177-86

F Mascarelli 1 , Y Courtois 1 , C Arruti 2

1 Unité de Recherches Gérontologiques. U. 118 INSERM, 29 rue Wilhem, 75016 Parix, France 2 Laboratorio de Cultivo de Tejidos, Departamento de Histologia y Embriologia, Facultad de Medicina, Avenida General Flores, 2125 Montevideo, Uruguay

DOI: 10.1016/s0014-4835(89)80068-5
PMID: 2924806
Pubmed: https://pubmed.ncbi.nlm.nih.gov/2924806
Texto completo: https://www.sciencedirect.com/science/article/abs/pii/S0014483589800685?via%3Dihub

Abstract:
When adult bovine lenses were cultured in vitro, the purified retina-derived growth factors EDGF I or EDGF II, as well as the soluble fraction of the retina RE, increased the rate of incorporation of [35S]methionine into protein in cells belonging to different populations in the anterior epithelium as well as in fibers from the most superficial region of the cortex. These fiber cells were the most sensitive to stimulation by the retinal factors as they exhibit a significant increase of total protein synthesis 24 hr after addition of the factors to the culture medium. The epithelial cells studied--central epithelial cells and germinative cells--appeared stimulated only 1 day later. The stimulation of incorporation was not directed towards a particular subset of proteins but to all major polypeptides constituting the electrophoretic pattern of each cell population. It is suggested that this type of ocular signal, which stimulates the expression of a definite program, may act as a permissive signal.

An eye-derived growth factor regulates epithelial cell proliferation in the cultured lens

Differentiation 1985 28(3):286-90

C Arruti 1 , A Cirillo 1 , Y Courtois 2

1 Laboratorio de Cultivo de Tejidos, Depto. de Histologia y Embriologia, Facultad de Medicina de Montevideo, Avda. Gral. Flores 2125, Montevideo, Uruguay 2 Unité de Recherches Gérontologiques, INSERM U. 118, CNRS ERA 842, Association Claude-Bernard, 29 rue Wilhem, Paris, France

DOI: 10.1111/j.1432-0436.1985.tb00837.x
PMID: 3996799
Pubmed: https://pubmed.ncbi.nlm.nih.gov/3996799
Texto completo: https://www.sciencedirect.com/science/article/abs/pii/S0301468111606762?via%3Dihub

Abstract:
Lenses in organ culture permit an analysis of factors acting on epithelial cell growth, while keeping the normal steric constraints of the cell population. By employing this technique with radioautography of epithelial whole mounts, we showed that the DNA synthesis found in the epithelia of cultured bovine lenses follows an organized spatial and temporal pattern during culture. Within the first 48 h, active cells were located at the preequatorial region ("germinative zone"), a distribution consistent with the in vivo spatial organization of multiplying cells. Starting at about 48 h, cells from the central region of the epithelium--a nonproliferating population--were triggered to synthesize DNA in the presence of eye-derived growth factor (EDGF). When cultured in serum-free medium, only a small fraction of the cells was labeled, but when a low serum concentration was present, this fraction reached 50% of the cell population. The stimulatory effect of EDGF required a lag period, but its effect reached a maximum exceeding that found for serum. However, the cells from the germinative region, having a cell density three- to four-fold higher than the central region, were not stimulated to proliferate. This occurred irrespective of the presence of EDGF or serum. If this growth-stimulatory activity derived from the retina were the actual factor controlling cell proliferation in the lens in vivo, then the results presented here would point to the presence of a regulatory mechanism similar to that known for some other hormones.

Hysterectomy of the newborn guinea-pig, subsequent effects on the oestrous cycle and life span of the corpora lutea

J Endocrinol 1975 66(2):233-7

W Buño, E Carlevaro, L Riboni, H D'Albora, L de los Reyes, D Zipitria, R Domínguez

DOI: 10.1677/joe.0.0660233
PMID: 1172521
Pubmed: https://pubmed.ncbi.nlm.nih.gov/1172521
Texto completo: https://joe.bioscientifica.com/doi/10.1677/joe.0.0660233

Abstract:
Longer oestrous cycles result from neonatal hysterectomy than from hysterectomy in adult life. Section and cauterization of the utero-vaginal union also prolonged the vaginal closure period up to an average of 55 days. The destruction of the mesometrium did not lengthen the oestrous cycle. Uterine autografts in hysterectomized newoborn guinea-pigs did not prevent the long cycles.