Publicaciones por año
Publicaciones del Departamento de Histología y Embriología
An eye-derived growth factor regulates epithelial cell proliferation in the cultured lens
Differentiation 1985 28(3):286-90
C Arruti 1 , A Cirillo 1 , Y Courtois 2
1 Laboratorio de Cultivo de Tejidos, Depto. de Histologia y Embriologia, Facultad de Medicina de Montevideo, Avda. Gral. Flores 2125, Montevideo, Uruguay 2 Unité de Recherches Gérontologiques, INSERM U. 118, CNRS ERA 842, Association Claude-Bernard, 29 rue Wilhem, Paris, France
DOI: 10.1111/j.1432-0436.1985.tb00837.x
PMID: 3996799
Pubmed: https://pubmed.ncbi.nlm.nih.gov/3996799
Texto completo: https://www.sciencedirect.com/science/article/abs/pii/S0301468111606762?via%3Dihub
Abstract:
Lenses in organ culture permit an analysis of factors acting on epithelial cell growth, while keeping the normal steric constraints of the cell population. By employing this technique with radioautography of epithelial whole mounts, we showed that the DNA synthesis found in the epithelia of cultured bovine lenses follows an organized spatial and temporal pattern during culture. Within the first 48 h, active cells were located at the preequatorial region ("germinative zone"), a distribution consistent with the in vivo spatial organization of multiplying cells. Starting at about 48 h, cells from the central region of the epithelium--a nonproliferating population--were triggered to synthesize DNA in the presence of eye-derived growth factor (EDGF). When cultured in serum-free medium, only a small fraction of the cells was labeled, but when a low serum concentration was present, this fraction reached 50% of the cell population. The stimulatory effect of EDGF required a lag period, but its effect reached a maximum exceeding that found for serum. However, the cells from the germinative region, having a cell density three- to four-fold higher than the central region, were not stimulated to proliferate. This occurred irrespective of the presence of EDGF or serum. If this growth-stimulatory activity derived from the retina were the actual factor controlling cell proliferation in the lens in vivo, then the results presented here would point to the presence of a regulatory mechanism similar to that known for some other hormones.
Monolayer organization by serially cultured bovine corneal endothelial cells: effects of a retina-derived growth-promoting activity
Exp Eye Res 1982 34(5):735-47
C Arruti 1 , Y Courtois 2
1 Laboratorio de Cultivo de Tejidos, Departmento de Histología y Embriología, Facultad de Medicina, Av. Gral Flores 2125, Montevideo, Uruguay 2 Unité de Recherches Gérontologiques U118, INSERM Développement et Sénescence Cellulaire, ERA 842, CNRS, 29 rue Wilhem, 75016 Paris, France
DOI: 10.1016/s0014-4835(82)80034-1
PMID: 7084337
Pubmed: https://pubmed.ncbi.nlm.nih.gov/7084337
Texto completo: https://www.sciencedirect.com/science/article/abs/pii/S0014483582800341?via%3Dihub
Abstract:
This study describes the ability of bovine corneal endothelial cells to proliferate and organize monolayers when cultured in the presence of a saline-soluble fraction of the retina (RE). This strongly stimulates cell proliferation by increasing the growth rate. In the continuous presence of RE, the cells organize a confluent monolayer composed of closely apposed polygonal cells lying on a basement membrane. Thus, over a large number of generations, these cells express most of their differentiated traits: contact inhibition of movement, proliferation arrest at monolayer formation, and the extracellular deposition of a material organized as a typical basement membrane. The presence of RE increases by a factor of 10 the number of cellular generations expressing these differentiated traits, and subcultured cells maintained in a medium lacking Re are unable to build up a similar organization at confluency, even if this is extended over several months. If these cultures are transferred to a medium containing RE, they behave like endothelial cells which have been always cultured with RE. We suggest that the expression of the endothelial differentiated state is related to the raised mitotic activity induced by the retinal factor.
Hysterectomy of the newborn guinea-pig, subsequent effects on the oestrous cycle and life span of the corpora lutea
J Endocrinol 1975 66(2):233-7
W Buño, E Carlevaro, L Riboni, H D'Albora, L de los Reyes, D Zipitria, R Domínguez
DOI: 10.1677/joe.0.0660233
PMID: 1172521
Pubmed: https://pubmed.ncbi.nlm.nih.gov/1172521
Texto completo: https://joe.bioscientifica.com/doi/10.1677/joe.0.0660233
Abstract:
Longer oestrous cycles result from neonatal hysterectomy than from hysterectomy in adult life. Section and cauterization of the utero-vaginal union also prolonged the vaginal closure period up to an average of 55 days. The destruction of the mesometrium did not lengthen the oestrous cycle. Uterine autografts in hysterectomized newoborn guinea-pigs did not prevent the long cycles.
'Advanced puberty' in female guinea pigs treated with human chorionic gonadotrophin (HCG) or testosterone enantate (TE) at birth
Horm Res 1974 5(6):344-50
H D'Albora, E Carlevaro, L Riboni, L de los Reyes, D Zipitría, R Domínguez
Departamento de Histología y Embriología, Facultad de Medicina, Montevideo
DOI: 10.1159/000178649
PMID: 4426573
Pubmed: https://pubmed.ncbi.nlm.nih.gov/4426573
Texto completo: https://doi.org/10.1159/000178649
Abstract:
Newborn female guinea pigs treated with HCG or testosterone enantate at birth display a ‘precocious puberty’ i.e., advanced vaginal opening and normal estrous cycles. Animals treated with a high dose of HCG (1,500 RU) had smaller ovaries than the controls or those treated with 500 RU. All groups had corpora lutea in their ovaries. Hemispaying reaction was 89.8% in 1,500-RU animals, 67.2% in the 500-RU group, and 37.2% in the controls. It is concluded that the ‘ovarian refractory period’ in the newborn guinea pig is not present under our experimental conditions. The influence of the ovarian steroid secretion on these results is discussed.
Steroid-producing cells in chick intersexual gonads
Gen Comp Endocrinol 1970 Feb;14(1):164-9
R Narbaitz, E M De Robertis Jr
a Cleft Palate Research Center, University of Pittsburgh USA b Departamento de Histología y Embriología, Facultad de Medicina, Montevideo, Uruguay
DOI: 10.1016/0016-6480(70)90020-1
PMID: 5461621
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5461621
Texto completo: https://linkinghub.elsevier.com/retrieve/pii/0016-6480(70)90020-1
Abstract:
Eggs of a sex-linked cross were injected on the fourth day of incubation with 100 μg of estradiol benzoate. Both injected and control chicks were sacrificed at varying intervals after hatching and a histochemical study of their gonads was conducted. Medullary cells, rich in lipids and with a high 3β-hydroxysteroid activity, were present in newly hatched inverted embryos with a localization similar to that found in normal female gonads. These characterstics persisted in the inverted gonad throughout the whole period of sex reversion. As these cells are supposed to secrete estrogens, it is suggested that the regression of cortex in inverted gonads is not due to lack of estrogen stimulation. Rapid proliferation and differentiation of testicular tissue in the medullary region takes place after the cortex has started its involution and appears to be a consequence of this process, rather than a cause.
Effects of estrogen or progesterone on prepuberal ovaries implanted in castrated adult male rats
Experientia 1969 25(7):754-5
Laura Riboni, Hortensia D'Albora, Emilia Carlevaro, R. Dominguez
Facultad de Medicina, Departamento de Histologia y Embriologia, Montevideo (Uruguay)
DOI: 10.1007/BF01897608
PMID: 5809308
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5809308
Texto completo: https://link.springer.com/article/10.1007/BF01897608
Abstract:
Des greffons d'ovaire de rat impubère placés dans la chambre antérieure de l'∄il du mâle castré, après traitement avec 0,5 mg de dipropionate d'∄stradiol ou avec deux doses de 1 mg de progestérone, ont montré une évidente ovulation. On interprète ce fait comme dû á l'action des hormones injectées dans le système nerveux central et qui favorisent le mécanisme de l'ovulation.
Effects of hemispaying on guinea pigs injected with testosterone on the first day of life
Acta Physiol Lat Am 1969 19(4):315-23
E Carlevaro, L Riboni, H D'Albora, R Domínguez, W Buño
DOI:
PMID: 5393647
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5393647
Texto completo:
Abstract:
Evolution of ovarian grafts in male guinea-pigs castrated the first day of life
Experientia 1968 24(5):459-60
R Domínguez, E Carlevaro, W Buño
Department of Histology and Embryology, School of Medicine, Montevideo (Uruguay)
DOI: 10.1007/BF02144390
PMID: 5674983
Pubmed: https://pubmed.ncbi.nlm.nih.gov/5674983
Texto completo: https://link.springer.com/article/10.1007/BF02144390
Abstract:
Postnatal evolution of steroidogenic cells in the chick ovary
Histochemie 1968 15(3):187-93
R Narbaitz, E M De Robertis Jr
Departamento de Histologla y Embriologia, Facultad de Medicina, Montevideo, Uruguay
DOI: 10.1007/BF00305882
PMID: 4246253
Pubmed: https://pubmed.ncbi.nlm.nih.gov/4246253
Texto completo: https://link.springer.com/article/10.1007/BF00305882
Abstract:
Summary. From hatching to sexual maturity, the left ovary of the chick was studied with cytochemical techniques for the demonstration of steroid synthesis. At the time of hatching, there are in the ovarian medulla cell groups loaded with lipids and cholesterol which give a positive reaction for Ah-3fl-hydroxysteroid dehydrogenase (Ah-3fl-HSDH). Because of the predominant growth of the cortex in the course of the postnatal morphogenesis of the ovary, these cells are shifted to the theca interna of the follicles and the interfollicular spaces (interstitial cells). The cytochemical tests used demonstrate that, at all the stages studied, there is steroid synthesis in these cells. From the 20th day on, granulosa cells show an unspecific enzymatic reaction, which is positive in the control sections incubated without substrate. A strong As-3fl-HSDH activity is observed in the granulosa cells of postovulatory follicles. The significance of these findings in relation to the life history of steroid producing cells in the embryonic and postnatal chick ovary is discussed.
SUCCINIC-DEHYDROGENASE ACTIVITY IN THE NEUROMUSCULAR SPINDLES OF THE CHICK
Experientia 1965 21:45-6
N I GERMINO, H D'ALBORA
Facultad de Medicina, Departamento de Histologia y Embriologia, Montevideo (Uruguay)
DOI: 10.1007/BF02136378
PMID: 14283529
Pubmed: https://pubmed.ncbi.nlm.nih.gov/14283529
Texto completo: https://link.springer.com/article/10.1007/BF02136378
Abstract: