Morphological evaluation of sperm from infertile men selected by magnetic activated cell sorting (MACS)

Posted by Ernesto on Tuesday, 3 June 2025
Autores: 
Gianni Curti 1 , Fernanda Skowronek 2 , Rita Vernochi 3 , Ana Laura Rodriguez-Buzzi 4 , Juan Carlos Rodriguez-Buzzi 5 , Gabriela Casanova 6 , Rossana Sapiro 7
Revista (o libro): 
Reprod Biol
Año: 
2014
Mes-dia: 
1200
issue, vol, paginas, etc: 
14(4):289-92
doi: 
10.1016/j.repbio.2014.07.002
PMID: 
25454495
Abstract: 
Electron microscopy analysis performed in five infertile human subjects after sperm selection by swim-up followed by magnetic activated cell sorting (MACS) demonstrated a decrease in the number of spermatozoa with characteristics compatible with cell death. However, no significant differences were found when the swim-up/MACS semen fraction was compared with swim-up fraction alone.
Afiliaciones: 
1 Departamento de Biologia Molecular - Instituto De Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, CP 11600 Montevideo, Uruguay. Electronic address: giannicurti@gmail.com. 2 Laboratorio de Biología Molecular de la Reproducción - Departamento de Histología y Embriología, Facultad de Medicina, Gral. Flores 2125, 11800 Montevideo, Uruguay. Electronic address: fernandaskowronek@gmail.com. 3 Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: draritaverno@gmail.com. 4 Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: anucharod@gmail.com. 5 Centro de Reproducción Humana del Interior (CERHIN), Bvar. Artigas 1665/102, Montevideo, Uruguay. Electronic address: jcrbuzzi@gmail.com. 6 Unidad de Microscopía Electrónica de Transmisión - Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: paragaby@gmail.com. 7 Laboratorio de Biología Molecular de la Reproducción - Departamento de Histología y Embriología, Facultad de Medicina, Gral. Flores 2125, 11800 Montevideo, Uruguay. Electronic address: rsapiro@fmed.edu.uy.
Enlace pubmed: 
https://pubmed.ncbi.nlm.nih.gov/25454495/
Enlace full text: 
https://linkinghub.elsevier.com/retrieve/pii/S1642-431X(14)00074-6
Cita: 
Curti G, Skowronek F, Vernochi R, Rodriguez-Buzzi AL, Rodriguez-Buzzi JC, Casanova G, Sapiro R. Morphological evaluation of sperm from infertile men selected by magnetic activated cell sorting (MACS). Reprod Biol. 2014 Dec;14(4):289-92. doi: 10.1016/j.repbio.2014.07.002. Epub 2014 Aug 8. PMID: 25454495.