Spatial and temporal distribution of Patched-related protein in the Drosophila embryo
Posted by Ernesto on Tuesday, 3 June 2025
Autores:
Carmen Bolatto 1 , Cristina Parada 2 , Fiorella Revello 3 , Alejandro Zuñiga 4 , Pablo Cabrera 5 , Verónica Cambiazo 6
Revista (o libro):
Gene Expr Patterns
Año:
2015
Mes-dia:
0900
issue, vol, paginas, etc:
19(1-2):120-8
doi:
10.1016/j.gep.2015.10.002
PMID:
26506022
Abstract:
Patched-related (Ptr) encodes a protein with 12 potential transmembrane domains and a sterol-sensing domain that is closely related in predicted topology and domain organization to Patched, the canonical receptor of the Hedgehog pathway. Here we describe the production of an antibody specific for Drosophila Ptr and analyse its spatial and temporal distribution in the embryo. We find that at early developmental stages Ptr is predominantly localized at cell periphery but later on it becomes strongly and almost exclusively expressed in hemocytes. Interestingly Ptr null mutant embryos died without hatching. Our findings suggest that Ptr plays an essential function in Drosophila development, perhaps as a new receptor of embryonic hemocytes.
Afiliaciones:
1 Laboratorio de Biología del Desarrollo, Departamento de Histología y Embriología, Facultad de Medicina-Universidad de la República, Montevideo, Uruguay. Electronic address: cbolatto@fmed.edu.uy.
2 Laboratorio de Biología del Desarrollo, Departamento de Histología y Embriología, Facultad de Medicina-Universidad de la República, Montevideo, Uruguay. Electronic address: cristinap@fmed.edu.uy.
3 Laboratorio de Biología del Desarrollo, Departamento de Histología y Embriología, Facultad de Medicina-Universidad de la República, Montevideo, Uruguay. Electronic address: fiorev85@gmail.com.
4 Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile and Fondap Center for Genome Regulation (CGR), Santiago, Chile. Electronic address: jano@inta.uchile.cl.
5 Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile and Fondap Center for Genome Regulation (CGR), Santiago, Chile.
6 Laboratorio de Bioinformática y Expresión Génica, INTA-Universidad de Chile and Fondap Center for Genome Regulation (CGR), Santiago, Chile. Electronic address: vcambiaz@inta.cl.
Enlace pubmed:
https://pubmed.ncbi.nlm.nih.gov/26506022/
Enlace full text:
https://linkinghub.elsevier.com/retrieve/pii/S1567-133X(15)30013-2
Cita:
Bolatto C, Parada C, Revello F, Zuñiga A, Cabrera P, Cambiazo V. Spatial and temporal distribution of Patched-related protein in the Drosophila embryo. Gene Expr Patterns. 2015 Sep-Nov;19(1-2):120-8. doi: 10.1016/j.gep.2015.10.002. Epub 2015 Oct 23. PMID: 26506022.