Rod precursors in the adult retina of the Austrolebias charrua annual fish
Posted by Ernesto on Wednesday, 4 June 2025
Autores:
M L Herrera 1 , S Silva 2 , I Berrosteguieta 2 , G Casanova 3 , J C Rosillo 4 , A S Fernández 5
Revista (o libro):
Tissue Cell
Año:
2023
Mes-dia:
0800
issue, vol, paginas, etc:
83:102150
doi:
10.1016/j.tice.2023.102150
PMID:
37423033
Abstract:
Rod photoreceptors in the adult teleost retina are produced by rod precursors located in the outer nuclear layer (ONL). Annual fishes of the genus Austrolebias exhibit extensive adult retinal cell proliferation and neurogenesis, as well as surprising adaptive strategies to their extreme and changing environment, including adult retinal plasticity. Thus, here we identify and characterize rod precursors in the ONL of the Austrolebias charrua retina. For this aim we used classical histological techniques, transmission electron microscopy, detection of cell proliferation, and immunohistochemistry. Through these complementary approaches, we describe a cell population clearly distinguishable from photoreceptors in the ONL of the adult retina of A. charrua, which we propose corresponds to the rod precursor population. These cells exhibited particular morphological and ultrastructural characteristics, uptake of cell proliferation markers (BrdU+) and expression of stem cell markers (Sox2+). Determining the existence of the population of rod precursors is crucial to understand the sequence of events related to retinal plasticity and regeneration.
Afiliaciones:
1 Departamento Neurociencias Integrativas y Computacionales, Lab. Neurobiología Comparada, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida. Italia 3318, 11600 Montevideo, Uruguay; Sección Fisiología y Nutrición, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.
2 Departamento Neurociencias Integrativas y Computacionales, Lab. Neurobiología Comparada, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida. Italia 3318, 11600 Montevideo, Uruguay.
3 Unidad de Microscopía Electrónica, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay.
4 Departamento Neurociencias Integrativas y Computacionales, Lab. Neurobiología Comparada, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida. Italia 3318, 11600 Montevideo, Uruguay; Departamento de Histología y Embriología, Facultad de Medicina, Universidad de la República, Avda. General Flores 2125, 11800 Montevideo, Uruguay. Electronic address: jrosillo@iibce.edu.uy.
5 Departamento Neurociencias Integrativas y Computacionales, Lab. Neurobiología Comparada, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida. Italia 3318, 11600 Montevideo, Uruguay; Laboratorio de Neurociencias, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay. Electronic address: afernandez@iibce.edu.uy.
Enlace pubmed:
https://pubmed.ncbi.nlm.nih.gov/37423033/
Enlace full text:
https://www.sciencedirect.com/science/article/abs/pii/S0040816623001386?via%3Dihub
Cita:
Herrera ML, Silva S, Berrosteguieta I, Casanova G, Rosillo JC, Fernández AS. Rod precursors in the adult retina of the Austrolebias charrua annual fish. Tissue Cell. 2023 Aug;83:102150. doi: 10.1016/j.tice.2023.102150. Epub 2023 Jun 28. PMID: 37423033.